Objective To study the effect of magnetic rewarming on the morphology and biomechanical properties of umbilical artery. Methods The vitrified umbilical artery was rewarmed by magnetothermal method and compared with the traditional water bath. The temperature distribution and thermal stress in the solution system were analyzed, and the rewarming effect was evaluated by tissue staining and mechanical test. Results (1) Compared with water bath rewarming, the temperature gradient ( =1.117±0.649℃) and thermal stress ( =0.026±0.016MPa) generated by magnetic rewarming were smaller, which could effectively avoid the occurrence of rupture and microcrack of umbilical artery, and the extracellular matrix, collagen fiber, elastic fiber and muscle fiber were evenly distributed. (2) The umbilical artery by magnetic rewarming had similar uniaxial tensile characteristic to the fresh group. There was no significant difference in elastic modulus and ultimate stress between the two groups (P>0.05), showing good elasticity and toughness. (3) The umbilical artery after water bath and magnetic rewarming showed different degrees of hardening, but the latter had the more similar stress relaxation and creep characteristics to the fresh umbilical artery. Conclusions Compared with water bath rewarming, magnetothermal method can effectively reduce the damage of rewarming stage, ensure the macroscopic, microscopic structure integrity of umbilical artery and better mechanical properties.These results provide important evidence for cryopreservation of large tissues or organs such as umbilical artery.