Objective To compare the effects of different support methods on eccentric control angle and muscle strength of knee joint in 10 weeks Nordic hamstring exercise. Methods Twenty-eight college male football players were randomly divided into hand support group (HS group, n = 14) and no hand support group (NHS group, n = 14) for 10 weeks. The eccentric control angle and eccentric muscle strength of knee joint were tested before and after the intervention. Results After 10 weeks of Nordic hamstring training: 1) the eccentric control flexion angle of knee joint in HS group and NHS group was significantly decreased by 8° and 10° (both P < 0.05), and the knee joint in NHS group was 12° lower than that in HS group (P < 0.05); 2) the peak torque of hamstring and quadriceps femoris in both HS and NHS groups was significantly increased, and the peak torque of hamstring centrifugal motion at 60°/s and 120°/s in NHS group was 16N and 13N higher than that in HS group, respectively (all P < 0.05). Conclusions NHS group is better than HS group in improving knee flexion Angle and centrifugal muscle force. It is suggested that Nordic hamstring training should be taken as one of the daily training for football players to prevent hamstring injury.