Periodontium is mechanoresponsive to multiple types of mechanical stimuli like occlusal and orthodontic force. It is widely used as loading subject in research regarding dental mechanical force model both in vivo and in vitro. This review summarizes various animal models and cell culture loading methods as well as parameters for periodontium in recent years, so as to provide reference for the study of periodontal mechanoresponsive mechanism and the development of new clinical therapies.