Objective Aiming at improving the biomechanical strength of the anastomotic stoma as well as reducing thermal tissue damage, a novel radiofrequency tissue welding electrode was developed. Methods A novel RF tissue welding electrode named Plum electrode with a hollow structure on the surface was designed and the Ring electrode was used as the control group to conduct the welding of intestinal tissue based on radiofrequency energy. Biomechanical properties of anastomotic stoma were studied by the indexes of burst pressure and shear test. The thermal characteristics of tissue during welding were investigated by electro-thermal-mechanical multi-field coupling analysis and thermocouple probe, and the tissue microstructure was also studied. Results In contrast to the Ring electrode group, a slightly higher mean strength is acquired with the shear strength and burst pressure results increasing from 9.71.47 N, 84.05.99 mmHg to 11.11.71 N, 89.46.60 mmHg respectively as well as a significant reduction in tissue thermal damage for the Plum electrode group, when the compression pressure of 20 kPa, RF energy of 120 W and welding duration of 8 s was applied to the target regions to achieve anastomosis. And histopathological examination observation demonstrates that intact and fully fused stomas can be formed in the anastomotic area. Conclusion The proposed novel electrode could reduce the thermal damage of tissue as well as improve the biomechanical strength of the anastomosis and thus achieve better fusion.