Ocular biomechanics, an increasingly vital field for elucidating the pathophysiological mechanisms of eye diseases, is garnering increasing attention. This review concisely summarizes a series of novel ocular biomechanical measurement techniques and their research advances in 2025, which enable multi-dimensional and precise quantification of ocular tissue mechanical properties. It also demonstrates and emphasizes how the integration of artificial intelligence with multi-modal data serves as an ‘accelerator’, enhancing the clinical understanding of ocular biomechanics. Furthermore, the review highlights recent key points in exploring the role of mechanical factors in the pathogenesis of major ocular diseases, including myopia, keratoconus, and glaucoma, offering new perspectives for identifying novel interventional targets. In the future, it is expected to evolve from ‘measuring forces’ to ‘modulating forces’, thereby paving the way for innovative therapeutic strategies in ophthalmology.