Objective This study focuses on real-world clinical scenarios and aims to quantify the biomechanical parameters of the One-Finger Zen Push Method applied to key acupoints in patients with cervical spondylosis, using patient comfort feedback as a reference. The goal is to elucidate the dynamic biomechanical characteristics of this technique during actual treatment. Methods The German Novel Pliance-X 32 Expert dynamic pressure distribution glove system was employed to record biomechanical parameters when Tuina practitioners applied the One-Finger Zen Push Method to Jianjing and Fengchi acupoints at force levels based on patient comfort feedback. A 1-minute segment of the mid-operation phase, representing stable force output, was selected for analysis. Key parameters included maximum force, mean force, peak pressure, average pressure, force-time integral (FTI), pressure-time integral (PTI), and operation frequency. Results The coefficient of variation (CV) for most parameters—maximum force, mean force, peak pressure, average pressure, FTI, and PTI—was below 20% for both acupoints, with operation frequency CV all below 10%, indicating high consistency and low variability in mechanical output. Except for frequency, all major parameters showed statistically significant differences between the two acupoints (P<0.01), suggesting site-specific biomechanical characteristics. Further subgroup analysis based on patient sex revealed no significant differences in any parameter across the two acupoints (P>0.05), indicating that senior-level Tuina practitioners maintain consistent mechanical performance regardless of patient sex, reflecting the technique's strong operational stability and individual adaptability. Conclusion This study objectively quantifies the biomechanical parameter ranges during real clinical practice. The One-Finger Zen Push Method, when performed by experienced Tuina practitioners, demonstrates stable, focused, and quantifiable biomechanical output. These findings confirm the reproducibility and measurable regularity of traditional clinical Tuina techniques within a modern biomechanical framework and provide scientific evidence for their teaching evaluation, clinical standardization, and inheritance.