: Objective To analyze the influence of different loads (abdominal pressure and uterine weight) on stress and deformation of uterine ligaments using the finite element method, and obtain the sensitivity of each accessory ligament to the changes of load on uterus. Methods The three-dimensional (3D) models of retroverted uterus and its accessory ligaments were established, loads and constraints were set in ABAQUS software, and the stress and deformation of uterine ligaments were calculated. Results The changes of abdominal pressure and uterine weight alone or both together would cause tensile deformation of uterine ligaments, and the deformation and stress increased with the load increasing. The deformation and stress were mainly distributed in the middle and lower part of each ligament or at the junction with uterus. The maximum stress and displacement were concentrated at the junction of each ligament with uterus or pelvic wall, and uterus weight had a greater influence on the ligaments than abdominal pressure. When abdominal pressure changed alone or together with uterine weight, the stress and sensitivity to load changes of the ligaments were in the order of uterosacral ligament (USL), broad ligament (BL), cardinal ligament (CL) and round ligament (RL). When uterine weight changed alone, the stress and sensitivity to load changes of the ligaments were in the order of USL>CL >RL >BL. The deformation and sensitivity to load changes of the ligaments remained unchanged and the order was BL>RL>USL>CL. Conclusions The sensitivity of each uterine ligament changing with uterine load was studied through finite element analysis, and the research results were consistent with the clinical data, which could provide guidance for optimization of surgical scheme of uterus prolapsed in clinic and exploration of pathogenesis.