Objective To explore the effects of wearing running shoes with different heel-to-toe drops on lower limb joint loading at the same running speed, so as to provide references for the design of running shoes and for runners to buy running shoes. Methods A total of 18 male runners completed the running test by wearing shoes with 0 mm and 10 mm heel-to-toe drops at the speed of (4. 0±0. 2) m / s, the kinematic parameters and ground reaction force (GRF) of lower limbs were collected synchronously by using infrared high-speed motion capture system and three-dimensional (3D) force plates. Statistical parameter mapping (SPM) was used to analyze the effects from heel-to-toe drop of running shoes on GRF as well as 3D moments of lower limb joints during stance phase. Results The heel-to-toe drop of running shoes had no effects on vertical GRF, but had significant effects on moment-time series of lower limb joints. Compared with wearing 0 mm heel-to-toe drop running shoes, wearing running shoes with 10 mm heel-to-toe drop increased the hip internal rotation moments in 27% -38% of stance phase and the knee extension moments in 47% -75% of stance phase, meanwhile decreased the plantar flexion moments, valgus moments and external rotation moments of ankle joints in 16% -33% , 25% -30% and 12% - 25% of the stance phase, respectively. Conclusions Compared with wearing 0 mm heel-to-toe drop running shoes, wearing running shoes with 10 mm heel-to-toe drop increases the hip joint loading, decreases the ankle joint loading in early stance phase, and increases the knee joint loading in middle stance phase. It is suggested that runners choose running shoes according to their own characteristics and the effects of heel-to-toe drop of running shoes on lower limb joint loading.