Objective This study aims to evaluate the mechanical and biomechanical properties of the novel NiTi memory alloy frame with matching bone graft for lumbar interbody fusion. Methods The mechanical properties of the NiTi memory alloy frame were tested. Moreover, cadaveric testing was conducted to compare the biomechanical properties between the NiTi memory alloy frame with matching bone graft and the traditional box-shape cage. Results The maximum compressive strength of the NiTi memory alloy frame was 12964±962N. The frame height decreased by 4.68±0.03mm with a recovery rate up to 99.86%. The NiTi memory alloy frame group presented a smaller ROM than did the intact lumbar model group, and no ROM difference compared with the box-shape cage group. Notably, in the models with unilateral/bilateral posterior fixation, the NiTi memory alloy frame with matching bone graft group showed the smallest ROM in vitro flexibility test (P < 0.05). The pull-out strength of the NiTi shape memory alloy frame with matching bone graft group was significantly stronger than that of the box-shape cage group (P < 0.05). NiTi memory alloy frame with matching bone graft method has excellent mechanical properties, and greatly improve the stability of surgical segment and the pullout strength of implants.