Objective Compare the biomechanical properties of four groups with different posterior monosegmental fixation and make the evaluation. Method Twenty four fresh frozen of one-week-old calf spines (T11-L3) were divided into four groups following the introduction of incomplete compression fracture at the vertebral body of L1 by the method of excision. Four types of monosegmental fixation were applied to the four groups respectively to restore spinal stability. A cyclic loading were applied to the specimens at rate of 1.0 Hz up to 3000 cycles in flexion/extension, left/right lateral bending and left/right rotation respectively. Segmental stability (T13-L1) tests were then performed on the specimens in the condition of intact, injured, fixation respectively. Range of motion(ROM)in flexion/extension, left/right axial rotation, left/right lateral bending were determined by three dimensional laser scanner. Then the ROM was standardized into stability potential index(SPI)to compare the stability in six loading directions of four types of fixation in four conditions. Result All of the four groups with monosegmental fixation could significantly increase the stability in six motion directions when compared to their injury condition(P<0.01), and significant superior than that of intact condition(P<0.01), but there was no significant difference among the four groups(P>0.05).The stability in six motion directions after cyclic loading was inferior when compared to that of before cyclic loading respectively, but there was no significant difference in between(P>0.05), or among the four groups(P>0.05). Conclusions As long as it could ensure to implant the pedicle screw into pedicle and vertabra, and avoid the fractured areas in the affected segment, the angle of pedicle screw directed to superior or inferior endplate will not affect both the instant and post-fatigue stability.