Objective To discuss the characteristics of flexor and extensor torque of the knee joint by carrying out isokinetic testing on women handball players in Guangdong Province, and to provide some isokinetic test data for the strength and physical training research on handball players. Methods Flexor and extensor of knee joint in fourteen players from Guangdong provincial handball team were measured by CON-TREX isokinetic testing system. Relative peak torque, hamstring/quadriceps ratio, total work, average power and fatigue index were selected to study the isokinetic characteristics of the women handball players’ knee joint. Independent t tests were employed to compare the results between flexor and extensor. Results For women handball players, at the velocity of 60°/s (low speed test)and 240°/s(high speed test), the relative extensor peak torque value was significantly greater than that of the flexor（P＜0.01）; at the velocity of 240°/s, the hamstring/quadriceps ratio was about 76%; at the velocity of 60°/s, the total work and average power of the extensor was greater than that of the flexor（P＜0.01）; at the velocity of 240°/s, the total work and average power of the extensor in left knee joint were greater than that of the flexor（P＜0.05）; the range of fatigue index was from 0 to 0.49. Conclusions The maximum strength and speediness strength of extensor was greater than that of the flexor; at the velocity of 240°/s, the hamstring/quadriceps ratio of the knee joint was relatively lower, which showed that the speediness strength of flexor in knee joint was relatively low; the speediness strength and the working capability of the extensor in knee joint was greater than that of the flexor; the fatigue index of extensor and flexor of knee joint was relatively low, which may indicate that the level of strength stamina of extensor and flexor in knee joint was relatively low.